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Teleradiology is a subset of telemedicine dealing with the transmission, display, and diagnosis from images, in addition to other patient-related information, between a remote examination site and an expert center. The technology requirement for teleradiology is more stringent than that of general telemedicine because the former involves images. Basically telemedicine without teleradiology requires only very simple technology: a computer gathers all necessary patient information, examination results, and diagnostic reports; arranges them in proper order with or without a standard format at the referring site; and transmits them through telecommunication technology to a second computer at the expert center where the information is displayed as a softcopy on the monitor. One important issue in telemedicine is patient privacy, which has to be considered seriously during the delivery of the telemedicine service. In modern hospitals or clinics, the information gathering and the arrangement of the information in proper order can be handled by the hospital information system

Figure 8-15: In 2D drawings such as this, you should consider various options, such as rotating the snap or creating a new UCS.

135-41

polymorphisms (STRPs; also known as microsatellites) are sequences of 3 5 base pairs repeated 10 30 times which have been found to be more evenly distributed through the genome than VNTRs A third type of marker is offered by sequence tagged sites (STSs), short sequences of 200 500 base pairs, which have a unique location in the genome The genome is cut into small pieces with restriction enzymes and each piece individually sequenced in a process called shotgun sequencing Regions of overlap allow the assembly of progressively longer sequences some of which will contain markers These markers are used to generate the physical map of the genome by gradually building up a hierarchical picture of its physical structure.

ASSOC returns a list that finds the entry associated with an item in a list. It takes two arguments: the item in the list and the list itself. For example, if you specify the group code (such as 10) as the first argument, it returns the code s value (which would be the start point of a line).Or, if a list named myobject contains three groups, as in ((0 . group 0 ) (1 1.0 2.0) (3 4.2 1.5 6.75)), then (assoc 1 myobject) would return (1 1.0 2.0). SUBST

By contrast, a second approach to assembling the genome (taken by Celera) was to eschew creation of maps and instead to apply shotgun sequencing to the whole genome by randomly shearing it into 2000 10 000 base pair fragments and sequencing these individually Although this approach had proven remarkably successful in viral and prokaryote genomes, which lack extensive repeat sequences, its application to eukaryote genomes was quite controversial initially In practice, the map-based and whole genome shotgun approaches are not mutually exclusive and combining the two approaches assembled the human genome Human DNA fragments (100 000 200 000 base pairs) were generated by restriction digestion and cloned into bacteria as a library of vectors called bacterial arti cial chromosomes (BACs) These can accommodate fragments as large as 250 000 base pairs, so a library of 20 000 BAC clones accommodated the entire genome.

Restriction mapping of individual fragments allowed identi cation of overlap and thus the clones could be ordered Each BAC clone was mapped onto human chromosomes using markers (VNTRs, TSRPs, STSs) as described above to generate a physical map Each BAC clone was then cut into smaller 2000 base pair pieces and sequenced at each end Piecing these sequences together assembled the sequence of the BAC clone by generating rst a contiguous clone map or contig and eventually a complete sequence The physical map allowed assembly of the BACs into the nal version of the genome Surprisingly, the human genome was found to contain signi cantly less genes than anticipated (25 000 30 000 rather than 50 000 100 000 as previously thought) and contains hundreds of bacterial genes apparently acquired by horizontal transfer during the vertebrate lineage.